全文获取类型
收费全文 | 3594篇 |
免费 | 301篇 |
国内免费 | 150篇 |
出版年
2023年 | 79篇 |
2022年 | 52篇 |
2021年 | 108篇 |
2020年 | 131篇 |
2019年 | 132篇 |
2018年 | 121篇 |
2017年 | 144篇 |
2016年 | 126篇 |
2015年 | 134篇 |
2014年 | 177篇 |
2013年 | 270篇 |
2012年 | 116篇 |
2011年 | 142篇 |
2010年 | 139篇 |
2009年 | 202篇 |
2008年 | 200篇 |
2007年 | 203篇 |
2006年 | 170篇 |
2005年 | 160篇 |
2004年 | 138篇 |
2003年 | 103篇 |
2002年 | 106篇 |
2001年 | 88篇 |
2000年 | 74篇 |
1999年 | 66篇 |
1998年 | 59篇 |
1997年 | 43篇 |
1996年 | 62篇 |
1995年 | 51篇 |
1994年 | 56篇 |
1993年 | 42篇 |
1992年 | 50篇 |
1991年 | 38篇 |
1990年 | 34篇 |
1989年 | 25篇 |
1988年 | 16篇 |
1987年 | 24篇 |
1986年 | 13篇 |
1985年 | 25篇 |
1984年 | 25篇 |
1983年 | 13篇 |
1982年 | 20篇 |
1981年 | 20篇 |
1980年 | 11篇 |
1979年 | 11篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1976年 | 5篇 |
1974年 | 3篇 |
1971年 | 2篇 |
排序方式: 共有4045条查询结果,搜索用时 15 毫秒
991.
992.
The bulk of experiments that study stressor effects on ecosystem functioning consider only individual functions one at a time, and such narrow focus may well bias our understanding of the overall impact on ecosystem functioning. We used data from six published experiments in which marine illuminated sediment systems were exposed to nutrient enrichment, toxicants, sedimentation and warming, either alone or in combination. Measured functions were primary production, community respiration, inorganic nitrogen and phosphorus fluxes, and autotrophic biomass. We calculated two indices of multifunctionality that simultaneously considered all six functions: (i) a weighted average level of the functions and (ii) the number of functions that simultaneously exceed a critical threshold level. Stressors affected individual functions both positively and negatively, but multifunctionality was generally unaffected by both single and joint stressors. The filtering capacity of coastal illuminated sediment systems thus appears resilient to exposure to moderate levels of multiple stressors, most probably due to the robustness of the benthic microalgal community. We recommend using a multifunctionality approach in future studies on cumulative stressor effects on ecosystem functioning, particularly when considering functions related to ecosystem services. 相似文献
993.
Sylvain Lanouette Vanessa Mongeon Daniel Figeys Jean‐François Couture 《Molecular systems biology》2014,10(4)
Large‐scale characterization of post‐translational modifications (PTMs), such as phosphorylation, acetylation and ubiquitination, has highlighted their importance in the regulation of a myriad of signaling events. While high‐throughput technologies have tremendously helped cataloguing the proteins modified by these PTMs, the identification of lysine‐methylated proteins, a PTM involving the transfer of one, two or three methyl groups to the ε‐amine of a lysine side chain, has lagged behind. While the initial findings were focused on the methylation of histone proteins, several studies have recently identified novel non‐histone lysine‐methylated proteins. This review provides a compilation of all lysine methylation sites reported to date. We also present key examples showing the impact of lysine methylation and discuss the circuitries wired by this important PTM. 相似文献
994.
Colin Ruprecht Takayuki Tohge Alisdair Fernie Cara L. Mortimer Amanda Kozlo Paul D. Fraser Norma Funke Igor Cesarino Ruben Vanholme Wout Boerjan Kris Morreel Ingo Burgert Notburga Gierlinger Vincent Bulone Vera Schneider Andrea Stockero Juan Pedro Navarro Frank Pudel Bart Tambuyser James Hygate Jon Bumstead Louis Notley Staffan Persson 《Journal of visualized experiments : JoVE》2014,(87)
995.
Víctor Rosas‐Guerrero Ramiro Aguilar Silvana Martén‐Rodríguez Lorena Ashworth Martha Lopezaraiza‐Mikel Jesús M. Bastida Mauricio Quesada 《Ecology letters》2014,17(3):388-400
The idea of pollination syndromes has been largely discussed but no formal quantitative evaluation has yet been conducted across angiosperms. We present the first systematic review of pollination syndromes that quantitatively tests whether the most effective pollinators for a species can be inferred from suites of floral traits for 417 plant species. Our results support the syndrome concept, indicating that convergent floral evolution is driven by adaptation to the most effective pollinator group. The predictability of pollination syndromes is greater in pollinator‐dependent species and in plants from tropical regions. Many plant species also have secondary pollinators that generally correspond to the ancestral pollinators documented in evolutionary studies. We discuss the utility and limitations of pollination syndromes and the role of secondary pollinators to understand floral ecology and evolution. 相似文献
996.
997.
998.
999.
Potential application of aqueous two‐phase systems and three‐phase partitioning for the recovery of superoxide dismutase from a clarified homogenate of Kluyveromyces marxianus 下载免费PDF全文
Jesús Simental‐Martínez Marco Rito‐Palomares Jorge Benavides 《Biotechnology progress》2014,30(6):1326-1334
Superoxide dismutase (SOD; EC 1.15.1.1) is an antioxidant enzyme that represents the primary cellular defense against superoxide radicals and has interesting applications in the medical and cosmetic industries. In the present work, the partition behavior of SOD in aqueous two‐phase systems (ATPS) (using a standard solution and a complex extract from Kluyveromyces marxianus as sample) was characterized on different types of ATPS (polymer–polymer, polymer–salt, alcohol–salt, and ionic liquid (IL)–salt). The systems composed of PEG 3350‐potassium phosphate, 45% TLL, 0.5 M NaCl (315 U/mg, 87% recovery, and 15.1‐fold purification) and t‐butanol‐20% ammonium sulfate (205.8 U/mg, 80% recovery and 9.8‐fold purification), coupled with a subsequent 100 kDa ultrafiltration stage, allowed the design of a prototype process for the recovery and partial purification of the product of interest. The findings reported herein demonstrate the potential of PEG‐salt ATPS for the potential recovery of SOD. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:1326–1334, 2014 相似文献
1000.
Partition behavior of CD133+ stem cells from human umbilical cord blood in aqueous two‐phase systems: In route to establish novel stem cell primary recovery strategies 下载免费PDF全文
Mirna González‐González Marco Rito‐Palomares Olivia Méndez Quintero 《Biotechnology progress》2014,30(3):700-707
Aqueous two‐phase systems (ATPS) represent a promising strategy for the recovery of CD133+ stem cells. This particular type of stem cells has great potential for research and clinical applications. Traditional [polyethylene glycol (PEG), dextran (DEX), and ficoll] and novel (Ucon) polymer–polymer ATPS were exploited to study the partitioning behavior of CD133+ stem cells and contaminants from human umbilical cord blood (HUCB). The aim of the study was to select conditions under which the product of interest and the contaminants concentrate in opposite phases. To accomplish this, three independent samples were tested: (1) enriched CD133+ sample, (2) whole HUCB (contaminants), and (3) complex sample (CD133+ stem cells and contaminants). The objective of this research was to evaluate the partition behavior of CD133+ in ATPS in route to establish the basis for the development of a novel and scalable purification bioprocess. In conclusion, the partitioning behavior of CD133+ stem cells and contaminants from complex samples was as follows: 59% of CD133+ stem cells fractionated to the top phase when employing ficoll 400,000–DEX 70,000 or 100% to the bottom phase with Ucon‐DEX 75,000 and PEG 8,000‐DEX 500,000 ATPS. In average, 35% of the contaminants partitioned to the top phase of the ficoll 400,000‐DEX 70,000 ATPS, 99% to the dextran rich phase of the Ucon‐DEX 75,000 systems and 97% to the bottom phase of the PEG 8,000‐DEX 500,000. Cell viability was at least 98% after ATPS recovery. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:700–707, 2014 相似文献